Data Data from the Cytochrome P Time Dependent Inhibition Single Point assay For the validation, a literature search was performed to identify a selection of compounds which were time dependent inhibitors of the main cytochrome P isoforms. These inhibitors were selected and were screened in triplicate on the plate on 3 separate occasions. The results show that there is a high level of consistency over a range of inhibition values. Figure 2 Discrimination between reversible and time dependent CYP3A4 inhibition by mibefradil using midazolam as substrate. Mibefradil is both a reversible and time dependent CYP3A4 inhibitor as it exhibits inhibitory potential in both the absence and presence of NADPH in the pre-incubation with the inhibition greater in the latter incubation.
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